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Volume 2 Issue 2
Apr.  2022
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Article Navigation >  Frigid Zone Medicine  > 2022  >  2(2): 109-118
Xiuxiu Wang, Bingjie Hua, Meixi Yu, Shenzhen Liu, Wenya Ma, Fengzhi Ding, Qi Huang, Lai Zhang, Chongwei Bi, Ye Yuan, Mengyu Jin, Tianyi Liu, Ying Yu, Benzhi Cai, Baofeng Yang. Altered expression profile of long non-coding RNAs during heart aging in mice[J]. Frigid Zone Medicine, 2022, 2(2): 109-118. doi: 10.2478/fzm-2022-0015
Citation: Xiuxiu Wang, Bingjie Hua, Meixi Yu, Shenzhen Liu, Wenya Ma, Fengzhi Ding, Qi Huang, Lai Zhang, Chongwei Bi, Ye Yuan, Mengyu Jin, Tianyi Liu, Ying Yu, Benzhi Cai, Baofeng Yang. Altered expression profile of long non-coding RNAs during heart aging in mice[J]. Frigid Zone Medicine, 2022, 2(2): 109-118. doi: 10.2478/fzm-2022-0015
shu

Altered expression profile of long non-coding RNAs during heart aging in mice

doi: 10.2478/fzm-2022-0015
  • 1.

    Department of Pharmacy at the Second Affiliated Hospital, and Department of Pharmacology at College of Pharmacy (the Key Laboratory of Cardiovascular Medicine Research, Ministry of Education), Harbin Medical University, Harbin 150086, China

  • 2.

    Institute of Clinical Pharmacy, Harbin Medical University, Harbin 150086, China

More Information
  • Corresponding author: Benzhi Cai, E-mail: caibz@ems.hrbmu.edu.cn; Baofeng Yang, E-mail: yangbf@ems.hrbmu.edu.cn

  • #These authors contributed equally.

  • Received Date: 2021-01-28
  • Accepted Date: 2021-06-28
    • Available Online: 2022-04-30
    Abstract
  •   Objective  Long noncoding RNAs (lncRNAs) play an important role in regulating the occurrence and development of cardiovascular diseases. However, the role of lncRNAs in heart aging remains poorly understood. The objective of this study was to identify differentially expressed lncRNAs in the heart of aging mice and elucidate the relevant regulatory pathways of cardiac aging.  Materials and methods  Echocardiography was used to detect the cardiac function of 18-months (aged) and 3-months (young) old C57BL/6 mice. Microarray analysis was performed to unravel the expression profiles of lncRNAs and mRNAs, and qRT-PCR to verify the highly dysregulated lncRNAs.  Results  Our results demonstrated that the heart function in aged mice was impaired relative to young ones. Microarray results showed that 155 lncRNAs were upregulated and 37 were downregulated, and 170 mRNAs were significantly upregulated and 44 were remarkably downregulated in aging hearts. Gene ontology analysis indicated that differentially expressed genes are mainly related to immune function, cell proliferation, copper ion response, and cellular cation homeostasis. KEGG pathway analysis showed that the differentially expressed mRNAs are related to cytokine-cytokine receptor interaction, inflammatory mediator regulation of TRP channels, and the NF-kappa B signaling pathway.  Conclusion  These results imply that the differentially expressed lncRNAs may regulate the development of heart aging. This study provides a new perspective on the potential effects and mechanisms of lncRNAs in heart aging.

     

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