Submission systemSubmit
Advanced Search
Volume 4 Issue 4
Oct.  2024
Turn off MathJax
Article Contents
Article Navigation >  Frigid Zone Medicine  > 2024  >  4(4): 224-232
Xianqi Sun, Yuzhen Li, Huiwen Yu, Jiaying Lin, Chen Wang, Quanlin Liu, Bingxue Bai. ISG15 promotes M5-induced hacat cell proliferation through Wnt signaling in psoriasis[J]. Frigid Zone Medicine, 2024, 4(4): 224-232. doi: 10.1515/fzm-2024-0022
Citation: Xianqi Sun, Yuzhen Li, Huiwen Yu, Jiaying Lin, Chen Wang, Quanlin Liu, Bingxue Bai. ISG15 promotes M5-induced hacat cell proliferation through Wnt signaling in psoriasis[J]. Frigid Zone Medicine, 2024, 4(4): 224-232. doi: 10.1515/fzm-2024-0022
shu

ISG15 promotes M5-induced hacat cell proliferation through Wnt signaling in psoriasis

doi: 10.1515/fzm-2024-0022
  • 1.

    Department of Dermetology, the Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China

  • 2.

    Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China

Funds:

the Natural Science Foundation of Heilongjiang Province PL2024H107

More Information
  • Corresponding author: Bingxue Bai, E-mail: bxbddd@163.com
  • Received Date: 2024-03-21
  • Accepted Date: 2024-04-25
    • Available Online: 2024-10-01
    Abstract
  •   Objective   Psoriasis is a common chronic, recurrent, immune-mediated inflammatory skin disease, which tends to occur in cold areas. Its pathogenesis is currently unclear. This study aims to screen differentially expressed genes in the psoriasis dataset, identify the central genes, detect the expression of central genes in psoriasis lesions of patients in the cold regions and then conduct further research.   Methods   Differential genes associated with psoriasis in the GEO database were analyzed, and functional enrichment analysis and protein-protein interaction network analysis. The expression results of the identified genes were validated in psoriasis cell models. The ISG15 gene, which showed the most significant difference in expression, was further studied. The expression level of ISG15 protein in psoriasis was examined. Then, we knocked out ISG15 in psoriasis cell models and detected keratinocyte proliferation by MTT, Real-Time PCR and Western Blot. Western Blot showed the expression of β-catenin after ISG15 gene knockout.   Results   We detected the protein expression of ISG15 in the cold area of Northeast China, and found that the expression of ISG15 increased in patients with psoriasis, and the proliferation of keratinocytes and the expression of β-catenin decreased in psoriasis cell model after ISG15 was knocked down. ISG15 regulates keratinocyte proliferation through Wnt signaling pathway in psoriasis.   Conclusions   ISG15 expression is increased in psoriatic cells and skin lesions of patients with psoriasis. In psoriasis, ISG15 promotes keratinocyte proliferation through the Wnt signaling pathway.

     

    • FullText(HTML)
  • loading
    • References(16)
  • [1]
    Mease P, Palmer J, Hur P, et al. Utilization of the validated psoriasis epidemiology screening tool to identify signs and symptoms of psoriatic arthritis among those with psoriasis: a cross-sectional analysis from the US-based corrona psoriasis registry. J Eur Acad Dermatol Venereol, 2019; 33(5): 886-892. doi: 10.1111/jdv.15443
    [2]
    De Simone C, Caldarola G, Moretta G, et al. Moderate-to-severe psoriasis and pregnancy: impact on fertility, pregnancy outcome and treatment perspectives. G Ital Dermatol Venereol, 2019; 154(3): 305-314.
    [3]
    Parisi R, Iskandar I, Kontopantelis E, et al. National, regional, and worldwide epidemiology of psoriasis: systematic analysis and modelling study. BMJ, 2020; 369: m1590.
    [4]
    Tokuyama M, Mabuchi T. New treatment addressing the pathogenesis of psoriasis. Int J Mol Sci, 2020; 21(20): 7488. doi: 10.3390/ijms21207488
    [5]
    Girolomoni G, Strohal R, Puig L, et al. The role of IL-23 and the IL-23/ T 17 immune axis in the pathogenesis and treatment of psoriasis. J Eur Acad Dermatol Venereol, 2017, 31(10): 1616-1626. doi: 10.1111/jdv.14433
    [6]
    Hawkes J E, Chan T C, Krueger J G. Psoriasis pathogenesis and the development of novel targeted immune therapies. J Allergy Clin Immunol, 2017; 140(3): 645-653. doi: 10.1016/j.jaci.2017.07.004
    [7]
    Yong L, Yu Y, Li B, et al. Calcium/calmodulin-dependent protein kinase IV promotes imiquimod-induced psoriatic inflammation via macrophages and keratinocytes in mice. Nature commun, 2022; 13(1): 4255. doi: 10.1038/s41467-022-31935-8
    [8]
    Juncker M, Kim C, Reed R, et al. ISG15 attenuates post-translational modifications of mitofusins and congression of damaged mitochondriain ataxia telangiectasia cells. Biochim Biophys Acta Mol Basis Dis, 2021; 1867(6): 166102. doi: 10.1016/j.bbadis.2021.166102
    [9]
    Perng Y C, Lenschow D J. ISG15 in antiviral immunity and beyond. Nat Rev Microbiol, 2018; 16(7): 423-439. doi: 10.1038/s41579-018-0020-5
    [10]
    Raposo R A, Gupta R, Abdel-Mohsen M, et al. Antiviral gene expression in psoriasis. J Eur Acad Dermatol Venereol, 2015; 29(10): 1951-1957. doi: 10.1111/jdv.13091
    [11]
    Gao L, Shen J, Ren Y, et al. Discovering novel hub genes and pathways associated with the pathogenesis of psoriasis. Dermatol Ther, 2020; 33(6): e13993.
    [12]
    Lu Y, Chen Y, Shi N, et al. L36G is associated with cutaneous antiviral competence in psoriasis. Front Immunol, 2022; 13: 971071. doi: 10.3389/fimmu.2022.971071
    [13]
    Ma J Y, Shao S, Wang G. Antimicrobial peptides: bridging innate and adaptive immunity in the pathogenesis of psoriasis. Chin Med J (Engl), 2020; 133(24): 2966-2975. doi: 10.1097/CM9.0000000000001240
    [14]
    Zhou Y, Wang P, Yan B X, et al. Quantitative proteomic profile of psoriatic epidermis identifies Oas2 as a novel biomarker for disease activity. Front Immunol, 2020; 11: 1432. doi: 10.3389/fimmu.2020.01432
    [15]
    Chen Y, Zhang Z, Zhang Y, et al. Gene set enrichment analysis and ingenuity pathway analysis to verify the impact of Wnt signaling in psoriasis treated with Taodan granules. Am J Transl Res, 2023; 15(1): 422-434.
    [16]
    Liu L, Zhou Y, Luo D, et al. Aberrant promoter methylation of Wnt inhibitory factor-1 gene is a potential target for treating psoriasis. Clin Immunol, 2023; 250: 109294. doi: 10.1016/j.clim.2023.109294
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(5)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (16) PDF downloads(1) Cited by()
    Proportional views
    Related

    Contact Us

    Editorial Office: Frigid Zone Medicine, Editorial Office of Frigid Zone Medicine, Heilongjiang Health Development Research Center, Zhongshan Road 112, Xiangfang District, Harbin, 150036, China

    Tel: 0451-87253028

    E-mail: editorialoffice@frigidzonemedicine.com

    Supported by: Beijing Renhe Information Technology Co., Ltd.    

    Links

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return